The lab claims fixation will cause the dyes to leak from the cells. I believe the idea is the fact that fixation may hurt cell membranes. I did find a paper which describes fixation utilizing methanol or acetic acid, just after staining that has a DCFDA by-product. DCFDA could be https://2-fdck-hcl51246.widblog.com/82945251/the-2-fdck-hcl-diaries